UASp regulatory sequences drive expression of ph-p containing mutations in the EH binding surface (amino acid replacement L1565R). The protein is tagged with Tag:FLAG.
wing disc, with Scer\GAL4en.PU
Expression of ph-pL1565R.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4en.PU results in grossly abnormal wing discs consistent with tissue overgrowth.