Zmynd10EY10866 mutants are viable and have no visible morphological defects. A climbing assay reveals that they are uncoordinated. This phenotype is not observed in revertant flies in which the P-element has been excised from the Zmynd10 locus.
No gross morphological defects are found in Ch neurons or terminal cilia in Zmynd10EY10866 mutants. Compartmentalization of the Ch neuron cilium also appears normal.
Homozygous Zmynd10EY10866 mutant males are infertile. In mutant males, the testes appear normal and contain developing sperm bundles. TEM shows that these sperm bundles generally consist of 64 sperm, suggesting that spermatocyte division and sperm differentiation are largely unaffected. However, motile sperm are not found as seminal vesicles are completely devoid of sperm, suggesting that the sperm are not transferred from the testes to the seminal vesicles.
Zmynd10EY10866 mutant sperm flagella exhibit a partial loss of dynein arms, with 12% showing axoneme splitting, whereby one or more doublet complexes become detached from the rest of the axoneme. in addition, ectopic luminal filaments (electron-dense cores) are also present within the 'A' microtubule of some doublets.
Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification.
Zmynd10EY10866 is rescued by Zmynd10Venus
Zmynd10EY10866 is partially rescued by Zmynd10V16G.Venus
Coordinated locomotion, fertility and sperm motility are completely restored in Zmynd10EY10866 mutants in a Zmynd10T:Avic\GFP-YFP.Venus background.
Fertility is partially restored in Zmynd10EY10866 mutants in a Zmynd10T:Avic\GFP-YFP.Venus background.