FlyBase Allele Report: Dmel\Doa[HMJ03121]

General Information

Symbol

Dmel\Doa^HMJ03121

Species

D. melanogaster

Name

FlyBase ID

FBal0292824

Feature type

allele

Associated gene

Dmel\Doa

Associated Insertion(s)

Carried in Construct

P{TRiP.HMJ03121}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMJ03121}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMJ03121

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

(Sopko et al., 2014)

female sterile, with Scer\GAL4^VP16.otu

(Yan and Perrimon, 2015)

lethal | female limited, with Scer\GAL4^dome-PG14

(Yan and Perrimon, 2015)

visible, with Scer\GAL4^nub-AC-62

(Yan and Perrimon, 2015)

Phenotype Manifest In

embryo | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Sopko et al., 2014)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

wing, with Scer\GAL4^nub-AC-62

(Yan and Perrimon, 2015)

Detailed Description

Statement

Reference

The expression of Doa^HMJ03121 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP.

(Ke et al., 2018)

Females heterozygous for Doa^HMJ03121 and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} crossed to Doa^HMJ03121 males results in: "few eggs,0% hatching"; egg/embryonic phenotype: "few eggs, abnormal"; maternal effect; oogenesis defect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

50903

y¹ v¹; P{TRiP.HMJ03121}attP40

Notes on Origin

Discoverer

Comments

Supplemental Dataset_S01.

(Yan and Perrimon, 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Doa^HMJ03121

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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