FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Avic\GFPAKAR2.UAS
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General Information
Symbol
Avic\GFPAKAR2.UAS
Species
A. victoria
Name
FlyBase ID
FBal0295387
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{UAS-AKAR2}
Also Known As
UAS-AKAR2
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Gervasi et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-AKAR2}
Cytology
Description

UASt regulatory sequences drive expression of the genetically encoded A-kinase activity reporter "AKAR2" (see PMID:16177793). This protein consists of two fluorescent proteins (ECFP and Citrine) that are separated by a phosphothreonine-binding domain (forkhead associated domain 1 (FHA1) from the Saccharomyces cerevisiae RAD53 gene) and an optimised protein kinase A (PKA) substrate domain (LRRATLVD). When phosphorylated by PKA, the PKA substrate domain interacts with the binding pocket of the FHA1 domain, increasing the fluorescence resonance energy transfer (FRET) between the two fluorophores.

(Gervasi et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Avic\GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Both aversive and appetitive short-term and long-term memory are normal in flies expressing Avic\GFPAKAR2.Scer\UAS under the control of Scer\GAL4238Y.

      (Gervasi et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Avic\GFPAKAR2.Scer\UAS
      Avic\GFPAKAR2.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (4)