UASt regulatory sequences drive expression of the bcd open reading frame (ORF), that is tagged at the C-terminal end with three copies of Tag:HA. The bcd ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or the C-terminal tag sequence.
wing | female, with Scer\GAL4Bx-MS1096
Expression of bcdScer\UAS.FRT5.FRT2.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in lethality in males and smaller wings than normal in females.