FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\brpSTaR.FRT.Stop.GFP
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General Information
Symbol
Dmel\brpSTaR.FRT.Stop.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0295695
Feature type
allele
Associated gene
Dmel\brp
Associated Insertion(s)
Carried in Construct
PBac{brp(FRT.Stop)GFP}
Also Known As
Brp-FSF-GFP
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Chen et al., 2014)
Progenitor genotype
Carried in construct
PBac{brp(FRT.Stop)GFP}
Cytology
Description

The PBac{brp(FRT.Stop)GFP} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an FRT cassette containing transcriptional and translational stop sequences, followed by a GFP tag, the 2A peptide and a lexA::VP16 fusion. The lexA::VP16 fusion contains a mutation in lexA that renders it non-functional. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of FLP recombinase the FRT cassette is excised. The brp protein produced is now tagged with GFP. Although the presence of the 2A peptide allows the lexA::VP16 fusion to be cotranslated as a separate protein, this protein is non-functional due to the mutation in lexA.

(Chen and Zipursky, 2014.4.16)
Allele components
Component
Use(s)
Regulatory region(s)
brp
Encoded product / tool
brp
Tagged with
GFP
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      This allele is part of the STaR system.

      (Chen et al., 2014)

      The PBac{brp(FRT.Stop)GFP} construct used in FBrf0223931 was originally constructed to have the structure

      (Chen and Zipursky, 2014.4.16)

      brp—FRT stop cassette—GFP—2A peptide— LexA::VP16

      (Chen and Zipursky, 2014.4.16)

      but the transformants were found to have a mutation in LexA rendering the protein nonfunctional; consequently, the 2A peptide— LexA::VP16 portion of the construct was not mentioned in the paper. The PBac{brp(-FRT)GFP} derivative obtained upon FLP-mediated recombination also carries sequences encoding the nonfunctional LexA::VP16 fusion protein.

      (Chen and Zipursky, 2014.4.16)

      PBac{brp-FRT-GFP-2A-LexA-VP16} was constructed as a control to show the results that would be obtained upon removal of the FRT cassette from PBac{brp(FRT.Stop)GFP}. The LexA::VP16 sequence was found to be unmutated upon transformation, so functional protein was produced.

      (Chen and Zipursky, 2014.4.16)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      brpSTaR.FRT.Stop.GFP
      brpScer\FRT.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (5)