FB2026_02 , released June 18, 2026
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Chen, Y., Zipursky, L. (2014.4.16). PBac{brp(FRT.Stop)GFP}. 
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FBrf0224700
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Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Stock Center by Yi Chen and Larry Zipursky, University of California at Los Angeles.
The PBac{brp(FRT.Stop)GFP} construct used in Chen et al. (FBrf0223931) was originally constructed to have the structure
brp-FRT stop cassette-GFP-2A peptide- LexA::VP16  
but the transformants were found to have a mutation in LexA rendering the protein nonfunctional; consequently, the 2A peptide- LexA::VP16   portion of the construct was not mentioned in the paper. The derivative obtained upon FLP-mediated recombination also carries sequences encoding the nonfunctional  LexA::VP16   fusion protein.
PBac{brp-FRT-GFP-2A-LexA-VP16} was constructed as a control to show the results that would be obtained upon removal of the FRT cassette from PBac{brp(FRT.Stop)GFP}. The   LexA::VP16   sequence was found to be unmutated upon transformation, so functional protein was produced.
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Research paper

Cell-type-Specific Labeling of Synapses In Vivo through Synaptic Tagging with Recombination.
Chen et al., 2014, Neuron 81(2): 280--293 [FBrf0223931]

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