amorphic allele - molecular evidence

P-element mediated recombination between P{SUPor-P}KG04646 and P{EPgy2}CG8613^EY06332 has resulted in deletion of part of the Shroom coding region, including exons 6 and 7. The 5' breakpoint is between 10,215,313 and 10,217,297 and the 3' breakpoint is between 10,238,326 and 10,239,743. Part of the sequence outside of the two P-elements, as well as sequences in the 5' coding region, are still present. The allele retains sequences immediately outside of the two P elements as well as sequences in the 5' coding region, likely because of gap repair. Sequences within the last three exons of Shroom, which encode the Rho-kinase-binding domain, are removed.