FB2026_03 , released September 17, 2026
Allele: Dmel\insbGFP
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General Information
Symbol
Dmel\insbGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0296583
Feature type
allele
Associated gene
Dmel\insb
Associated Insertion(s)
Carried in Construct
PBac{insb-GFP}
Key Links
Transgenic product class
genomic_DNA
(Coumailleau and Schweisguth, 2014)
wild_type
(Coumailleau and Schweisguth, 2014)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Coumailleau and Schweisguth, 2014)
wild_type
(Coumailleau and Schweisguth, 2014)
Mutagen
in vitro construct
(Coumailleau and Schweisguth, 2014)
Progenitor genotype
Carried in construct
PBac{insb-GFP}
Cytology
Description

A genomic fragment that encompasses the insb locus, including 6148bp of sequence 5' to the transcription start site through to 15129bp downstream of the 3'UTR (derived from the CH322-168B11 BAC clone). The coding region is tagged with GFP.

(Coumailleau and Schweisguth, 2014)
Allele components
Component
Use(s)
Regulatory region(s)
insb
Encoded product / tool
insb
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      epitope tag
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      pupal stage P5
      external sensory organ precursor cell IIa of mesonotum

      Comment: detected early then lost

      (Coumailleau and Schweisguth, 2014)
      external sensory organ precursor cell IIb of mesonotum

      Comment: later restricted to neuron

      (Coumailleau and Schweisguth, 2014)
      Additional Information
      Statement
      Reference

      GFP is detected in SOPs of the pupal notum. After SOP division, it is detected in pIIa and pIIb cells and is later restricted to neurons.

      (Coumailleau and Schweisguth, 2014)
       
      Marker for
      Reflects expression of
      insb
      (Coumailleau and Schweisguth, 2014)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The bristle loss phenotype seen in HE31/+ mutant flies is enhanced by loss of insb (using insbΔ1, in which the function of CG14478, Tes and qkr54B has been restored using P{CG14478-Tes-qkr54B-mCherry} (which comprises of the CG14478T:Disc\RFP-mCherry, TesT:Disc\RFP-mCherry and qkr54BT:Disc\RFP-mCherry alleles). Expression of insbT:Avic\GFP rescues this phenotype, returning the number of transformed macrochaetae to levels seen in HE31 heterozygotes alone.

      (Coumailleau and Schweisguth, 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      insbGFP
      insbT:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)