FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\lin-28dF30
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General Information
Symbol
Dmel\lin-28dF30
Species
D. melanogaster
Name
FlyBase ID
FBal0298041
Feature type
allele
Associated gene
Dmel\lin-28
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Sreejith et al., 2019)
Mutagen
P-element activity
(Stratoulias et al., 2014)
Progenitor genotype
P{EP}lin-28EP915
(Stratoulias et al., 2014)
Cytology
Description

Imprecise excision of the P{EP}lin-28EP915 progenitor insertion, resulting in 1007bp deletion upstream of the original insertion site (nucleotides 1242-2249 relative to the lin-28 transcription start site are deleted).

(Stratoulias et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:5,655,924..5,656,931 [+]
Comment:

1007 bp deletion resulting from the imprecise excision of P{EP}lin-28EP915.The deletion is reported at coordinates 1242-2249 relative to the lin-28 transcription start site. Bases on a 2702 nt reported toatl length of the transcript, 3L:5654683 was used as the tx start site. This is two bases downstream from the R6.04 annotated start.

(Stratoulias et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      lin-28dF30/lin-28dF30 pupae show significantly reduced volume and weight, but no change in the timing of pupariation compared to wild-type controls.

      lin-28Δ1/lin-28dF30 animals start pupariation earlier and have significantly reduced pupal volume and weight compared to wild-type controls, but no change in timing of eclosion from the start of metamorphosis.

      (González-Itier et al., 2018)

      Up to 50% of homozygous adults either fail to eclose or die shortly after eclosion.

      Homozygous adults show significantly reduced spontaneous and stimulated locomotor activity compared to controls.

      Newly eclosed homozygous males (0 to 3 hours after eclosion) show a loss of the transient dorsal internal oblique muscles. 8-day old homozygous males show minor muscle defects, with a shorter than normal muscle of Lawrence and larger than normal dorsal muscles.

      Homozygous females show a significantly reduced egg laying compared to controls. 32% of homozygous germaria show an increase in the number undifferentiated germline stem cells

      compared to controls. 75.4% of stage 7-10 egg chambers are abnormal, containing either more than the normal 15 nurse cells or showing degeneration. The additional nurse cell phenotype appears to be due to both egg chamber fusion and a mitotic defect (the number of nurse cells seen varies between 22 and 47 and egg chambers containing additional developing oocytes are seen).

      (Stratoulias et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      lin-28dF30
      (González-Itier et al., 2018)
      lin28dF30
      (Stratoulias et al., 2014)
      lin28df30
      (Sreejith et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)