FlyBase Allele Report: Dmel\wg[KO.NRT-wg]

General Information

Symbol

Dmel\wg^KO.NRT-wg

Species

D. melanogaster

Name

FlyBase ID

FBal0298268

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

TI{TI}wg^KO.NRT-wg

Carried in Construct

Also Known As

wg{KO; NRT-Wg}

Key Links

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Alexandre et al., 2014)

Progenitor genotype

wg^KO

(Alexandre et al., 2014)

Associated Insertion(s)

TI{TI}wg^KO.NRT-wg

Cytology

Description

Re-integration of DNA into the attP site present in wg^KO. The reintegrated sequence is as follows: 1. 'Nrt-wg' (a membrane-tethered, Tag:HA-tagged form of wg in which Tag:M(Nrt) acts as the membrane tethering sequence), and 2: a loxP cassette containing a w^+* marker. In addition, a recombined attP/B site is present 5' to the reintegrated sequence.

(Alexandre et al., 2014)

Allele components

Component

Use(s)

Tagged with

Tag:HA

epitope tag

Tag:M(Nrt)

membrane protein localization tag

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Alexandre et al., 2014)

partially lethal | conditional

(Alexandre et al., 2014)

Phenotype Manifest In

wing

(Alexandre et al., 2014)

wing margin taste bristle | decreased number

(Magny et al., 2021)

Detailed Description

Statement

Reference

The wings of homozygous flies have a slightly smaller surface area than those of control siblings. The arrangement of the wing margin bristles appears normal. The patterning of the microchaetae and macrochaetae on the dorsal thorax is normal.

On average, wg^l-17 clones induced in the wing disc in a wg^KO;NRT-wg homozygous background are smaller than wg^l-17 clones induced in the wing disc in a wild-type background.

(Alexandre et al., 2014)

When grown without overcrowding, homozygous flies are viable and have normally patterned appendages and other cuticular structures. When grown together with control siblings, homozygous flies show a reduced eclosion rate

Homozygous larvae show a developmental delay, entering pupariation later than controls.

(Alexandre et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

wg^KO.NRT-wg has wing margin taste bristle | decreased number phenotype, non-enhanceable by peg^Del1/peg^Del1

(Magny et al., 2021)

NOT suppressed by

Statement

Reference

wg^KO.NRT-wg has wing margin taste bristle | decreased number phenotype, non-suppressible by peg^UAS.EGFP/Scer\GAL4^69B

(Magny et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

wg KO

(Simões et al., 2022)

wg(KO; NRT-Wg)

(Xu et al., 2018)

wg^KO.NRT-wg

wg^KO;NRT-wg

wg^NRT

(Magny et al., 2021)

wg{KO; NRT-Wg;mini-white}

(Alexandre et al., 2014)

wg{KO; NRT-Wg}

(Simões et al., 2022, Alexandre et al., 2014)

wg{KO;NRT-Wg}

(Alexandre et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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