FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\H3-3AK27M.UAS.Tag:FLAG,Tag:HA
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General Information
Symbol
Hsap\H3-3AK27M.UAS.Tag:FLAG,Tag:HA
Species
H. sapiens
Name
FlyBase ID
FBal0298843
Feature type
allele
Associated gene
Hsap\H3-3A
Associated Insertion(s)
Carried in Construct
PBac{UAS-H3.3.K27M.FLAG.HA}
Key Links
Transgenic product class
missense_variant
(Herz et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Herz et al., 2014)
Mutagen
in vitro construct
(Herz et al., 2014)
Progenitor genotype
Carried in construct
PBac{UAS-H3.3.K27M.FLAG.HA}
Cytology
Description

UASt regulatory sequences drive expression of a K27M mutant of Hsap\H3-3A with C-terminal Tag:FLAG and Tag:HA flags.

(Herz et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\H3-3A
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  brain glioma
      CEA
      (Herz et al., 2014)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  brain cancer
      modeled by prosJF02308
      (Gaultier et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Herz et al., 2014)
      H3-3A:p.Lys28Met
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      H3-3A:p.Lys27Met
      Associated human disease model(s)
      External database links
      ClinVar: H3-3A_376435
      Comments concerning this variant

      Alternate designation K27M: the histone 3 polypeptide undergoes co-translational N-terminal methionine cleavage, thus the functional protein is one amino acid shorter.

      (FlyBase curators, 2019-)
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Hsap\H3F3AK27M.Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4GMR35E01 exhibit gross morphological defects, such as severe leg malformations and fusion phenotypes, and malformed, reduced or missing proboscis. The mutant flies die around eclosion.

      (Herz et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\H3-3AK27M.UAS.Tag:FLAG,Tag:HA
      Hsap\H3F3AK27M.Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1
      Hsap\H3F3AK27M.UAS.Tag:FLAG,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (5)