FB2026_03 , released September 17, 2026
Allele: Crei\ChR2T159C.UAS
Open Close
General Information
Symbol
Crei\ChR2T159C.UAS
Species
C. reinhardtii
Name
FlyBase ID
FBal0298845
Feature type
allele
Associated gene
Crei\ChR2
Associated Insertion(s)
Carried in Construct
PBac{UAS-ChR2.T159C}
Also Known As
UAS-ChR2T159C
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Dawydow et al., 2014)
Progenitor genotype
Carried in construct
PBac{UAS-ChR2.T159C}
Cytology
Description

UAS regulates expression of ChR2(T159C).

(Dawydow et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ChR2(T159C)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When retinal is not added to the food, larvae expressing Crei\ChR2T159C.Scer\UAS via Scer\GAL4RapGAP1-OK6 display no discernible reaction to photostimulation, as measured by the degree of immobilization. When retinal is added, light application induces reversible muscle paralysis, with the duration of immobilization scaling with light intensity.

      Scer\GAL4RapGAP1-OK6-mediated Crei\ChR2T159C.Scer\UAS expression results in light-evoked excitatory postsynaptic potentials (EPSPs) that are locked to the light stimulus. The frequency of EPSPs scales with light intensity.

      Even high intensity light fails to immobilize adult flies expressing Crei\ChR2T159C.Scer\UAS via Scer\GAL4RapGAP1-OK6.

      Scer\GAL4Gr5a.8.5-mediated Crei\ChR2T159C.Scer\UAS expression triggers a proboscis extension reflex (PER) in satiated flies using low light intensities.

      Scer\GAL4ple.PF Crei\ChR2T159C.Scer\UAS flies fail to acquire an aversive short-term memory for an odor paired with blue-light activation, even with dietary retinal supplementation.

      (Dawydow et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Crei\ChR2T159C.Scer\UAS
      Crei\ChR2T159C.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)