UAS regulatory sequences are fused upstream of a primary open reading frame (ORF1) encoding full-length mCherry, followed by two stop codons and then a second open reading frame (ORF2) encoding Ecol\dam tagged with Tag:MYC. The presence of ORF1 greatly reduces the translation of the Ecol\dam ORF2.
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Expression of Ecol\damUAS.LT3.Tag:MYC under the control of Scer\GAL4path-c135 does not lead to any changes in membrane morphology of carpet glia cells in the eye disc of third instar larvae when compared to controls.