A genomic fragment containing approximately 1.0kb upstream sequence and the open reading frame of tbrd-2 is followed by EGFP and 6xTag:polyHis tags and approximately 0.5kbp of tbrd-2 downstream sequence including the 3'UTR.
Expression of tbrd-2T:Avic\GFP-EGFP,T:Zzzz\His6 rescues the sterility and spermatid defects seen in tbrd-2HR mutant males.