FlyBase Allele Report: Dmel\azot[DsRed(T4)]

General Information

Symbol

Dmel\azot^DsRed(T4)

Species

D. melanogaster

Name

FlyBase ID

FBal0302677

Feature type

allele

Associated gene

Dmel\azot

Associated Insertion(s)

Carried in Construct

P{azot-RFP.M}

Key Links

Transgenic product class

wild_type

(Merino et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Merino et al., 2015)

Mutagen

in vitro construct

(Merino et al., 2015)

Progenitor genotype

Carried in construct

P{azot-RFP.M}

Cytology

Description

Disc\RFP expression is driven by 3kb upstream plus the full exon of azot.

(Merino et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

azot

Encoded product / tool

azot

Tagged with

DsRed(T4)

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Merino et al., 2015)

increased cell death

(Merino et al., 2015)

long lived

(Merino et al., 2015)

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Phenotype Manifest In

adult brain

(Merino et al., 2015)

Detailed Description

Statement

Reference

Adulthood-only expression of azot^DsRed(T4) under the combined control of Scer\GAL4^{elav.Switch.PO} and RU486 feeding decreases adult lifespan.

(Coelho et al., 2018)

Expression of azot^T:Disc\RFP in a wild type background increases the rate the apoptotic cell elimination normally seen in the 'loser' cells when cell competition is induced through generation of super-competitive dm^αTub84B.PBb-expressing clones.

The brains of 1 and 7-day-old flies expressing azot^T:Disc\RFP contain fewer vacuoles than controls.

Flies expressing azot^T:Disc\RFP show an increase in median survival and maximum lifespan compared to controls.

(Merino et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

azot^DsRed(T4), Scer\GAL4^{elav.Switch.PO} is an enhancer of abnormal neuroanatomy | adult stage | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^DsRed(T4), Scer\GAL4^{elav.Switch.PO} is an enhancer of short lived | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^DsRed(T4), Scer\GAL4^{elav.Switch.PO} is an enhancer of abnormal behavior | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Suppressor of

Statement

Reference

azot^DsRed(T4) is a suppressor of abnormal neuroanatomy | adult stage | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^DsRed(T4) is a suppressor of abnormal behavior | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^DsRed(T4) is a suppressor of abnormal memory | adult stage | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^DsRed(T4), Scer\GAL4^GMR.PF is a suppressor of increased cell death | larval stage phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

azot^DsRed(T4), Scer\GAL4^GMR.PF is a suppressor of increased cell death | adult stage phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

azot^DsRed(T4), Scer\GAL4^{elav.Switch.PO} is an enhancer of adult brain | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Suppressor of

Statement

Reference

azot^DsRed(T4) is a suppressor of adult brain | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^DsRed(T4), Scer\GAL4^GMR.PF is a suppressor of eye disc phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

azot^DsRed(T4), Scer\GAL4^GMR.PF is a suppressor of adult optic lobe phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

azot^DsRed(T4) is rescued by azot^DsRed(T4)

(Merino et al., 2015)

Rescues

azot^DsRed(T4) rescues azot^DsRed(T4)

(Merino et al., 2015)

Comments

The loss of 'loser' cell elimination seen in azot^KO mutant cells following dm-induced cell competition is rescued upon expression of azot^T:Disc\RFP.

Expression of two copies of azot^T:Disc\RFP rescues the wing defects seen in homozygous azot^KO mutant flies.

Expression of two copies of azot^T:Disc\RFP rescues the increase in vacuoles seen in azot^KO mutant brains.

Expression of two copies of azot^T:Disc\RFP rescues the reduced lifespan seen in azot^KO mutant brains.

(Merino et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

azot^DsRed(T4)

azot^T:Disc\RFP

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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