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General Information
Symbol
Dmel\JhI-26UAS.cLa
Species
D. melanogaster
Name
FlyBase ID
FBal0302986
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of JhI-26-RA coding sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Male flies overexpressing JhI-26Scer\UAS.cLa in testes under the control of Scer\GAL4nos.PU or Scer\GAL4Act.PU, when mated with wild-type females generate eggs which a hatch rate significantly lower than in control flies. Eggs from JhI-26Scer\UAS.cLa-expressing males mated with Wolbachia-infected females do not exhibit a decrease in hatch rate, indicating that the presence of Wolbachia has 'rescued' the JhI-26 overexpression phenotype.

Embryos from JhI-26Scer\UAS.cLa-expressing males and wild-type females reach late embryogenesis and develop a cuticle before dying. However, these cuticles consistently display head defects, sch as defects in the cephalopharyngeal apparatus. Around 67% of embryos display missing abdominal segments, or an internalized telson.

Embryos from wild-type females mated with JhI-26Scer\UAS.cLa-overexpressing males display numerous abnormalities, including asynchronous cleavage, unevenly distributed nuclei and different sizes of the nuclei. In individual embryos, three highly correlated major defects are observed. First, smaller and narrower metaphase spindles appear in the syncytial blastoderms, which are very similar to haploid metaphase spindles. Second, centrosomes often dissociate from mitotic spindles. Thirdly, the spindles exhibit a strange morphology with a regular couple of tubulin-containing particles in the middle of the divided chromosomes at telophase.

Like Wolbachia-infected males, JhI-26Scer\UAS.cLa-expressing males (under the control of Scer\GAL4nos.PU or Scer\GAL4Act.PU) may inhibit the receptivity to remating in mated wild-type females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
JhI-26Scer\UAS.cLa
JhI-26UAS.cLa
Name Synonyms
Secondary FlyBase IDs
    References (1)