FlyBase Allele Report: Hsap\RELA[AD.R35B12]

General Information

Symbol

Hsap\RELA^AD.R35B12

Species

H. sapiens

Name

FlyBase ID

FBal0304514

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R35B12-p65.AD}

PBac{R35B12-p65.AD}

Also Known As

R35B12-p65ADZp

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R35B12-p65.AD}

PBac{R35B12-p65.AD}

Cytology

Description

The R35B12 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR35B12

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuroblast NB7-2

Comment: when combined with P{R28H10-GAL4.DBD}

(Lacin and Truman, 2016)

adult stage

alpha'/beta' anterior-posterior Kenyon cell | intense

Comment: when combined with P{R26E07-GAL4.DBD} (combination referred to as 'MB461B')

when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB463B')

(Vogt et al., 2016, Aso et al., 2014)

alpha'/beta' middle Kenyon cell

Comment: when combined with P{R26E07-GAL4.DBD} (combination referred to as 'MB461B')

when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB463B')

(Vogt et al., 2016, Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4^DBD.R26E07 and Hsap\RELA^AD.R35B12 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

(Vogt et al., 2016)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R35B12 significantly decreases the amount of sleep in adult males or females, compared to controls, and results in extra rebound sleep after activation, but does not significantly affect locomotor activity of awake adult males; activation of neurons targeted by Scer\GAL4^DBD.R26E07 and Hsap\RELA^AD.R35B12 significantly decreases the amount of sleep in adult females, but not males, compared to controls, with no effect on locomotor activity of awake adult males; activation of neurons targeted by Scer\GAL4^DBD.R34A03 and Hsap\RELA^AD.R35B12 significantly decreases the amount of sleep in males, but not females, compared to controls, and significantly decreases locomotor activity of awake adult males, compared to controls.