FlyBase Allele Report: Dmel\Act57B[K326Q.K328Q.UAS]

General Information

Symbol

Dmel\Act57B^{K326Q.K328Q.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0304844

Feature type

allele

Associated gene

Dmel\Act57B

Associated Insertion(s)

Carried in Construct

P{UAS-Act57B.K326Q.K328Q}

Key Links

Transgenic product class

missense_variant

(Viswanathan et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Viswanathan et al., 2015)

Mutagen

in vitro construct

(Viswanathan et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-Act57B.K326Q.K328Q}

Cytology

Description

UASt regulatory sequences drive expression of an acetyl-mimetic mutant of Act57B carrying amino acid substitutions K326Q and K328Q.

(Viswanathan et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Act57B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^Mef2.PR

(Viswanathan et al., 2015)

flightless, with Scer\GAL4^Act88F.PB

(Viswanathan et al., 2015)

flightless, with Scer\GAL4^Mef2.PR

(Viswanathan et al., 2015)

majority die during P-stage, with Scer\GAL4^Mef2.PR

(Viswanathan et al., 2015)

partially lethal - majority die, with Scer\GAL4^Mef2.PR

(Viswanathan et al., 2015)

visible, with Scer\GAL4^Mef2.PR

(Viswanathan et al., 2015)

Phenotype Manifest In

indirect flight muscle cell, with Scer\GAL4^Act88F.PB

(Viswanathan et al., 2015)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Viswanathan et al., 2015)

Detailed Description

Statement

Reference

Expression of Act57B^{K326Q.K328Q.Scer\UAS} using Scer\GAL4^Mef2.PR predominantly results in pupal lethality with relatively few adult flies emerging from their puparia. The flight ability of the escapers is abolished and they show a characteristic "wings up" phenotype commonly associated with hypercontractile and damaged indirect flight muscles (IFMs). Indeed, polarized light microscopy reveals the absence of continuous IFM fibers.

Flies expressing Scer\GAL4^Mef2.PR>Act57B^{K328Q.Scer\UAS} have strikingly reduced climbing capabilities compared with wild-type.

Scer\GAL4^Act88F.PB-mediated expression of Act57B^{K326Q.K328Q.Scer\UAS} causes a complete loss of flight. These flies show prominently hypercontracted IFM fibers, characterized by separation and accumulation of fiber material at one or both attachment sites.

(Viswanathan et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Act57B^{K326Q.K328Q.Scer\UAS}

Act57B^{K326Q.K328Q.UAS}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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