FlyBase Allele Report: Dmel\mir-2a-1[sponge.V2.UAS.mCherry]

General Information

Symbol

Dmel\mir-2a-1^{sponge.V2.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0304966

Feature type

allele

Associated gene

Dmel\mir-2a-1

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.mir-2a.sponge.V2}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fulga et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-mCherry.mir-2a.sponge.V2}

Cytology

Description

Designed to knock-down the endogenous activity of both mir-2a-1 and mir-2a-2.

(Cook and Van Vactor, 2015.6.15)

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

(Fulga et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-2a-1

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

partially lethal - majority die, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

wing vein, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

Detailed Description

Statement

Reference

Scer\GAL4^tub.PU-mediated expression of P{UAS-mCherry.mir-2a.sponge.V2} results in a wing vein patterning defect and decreased wing size (in surviving adults).

(Fulga et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

61366

w^*; P{UAS-mCherry.mir-2a.sponge.V2}attP40; P{UAS-mCherry.mir-2a.sponge.V2}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-2a-1^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry}

mir-2a-1^{sponge.V2.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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