FlyBase Allele Report: Dmel\mir-276b[b.sponge.V2.UAS.mCherry]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\mir-276b^{b.sponge.V2.UAS.mCherry}

General Information

Symbol

Dmel\mir-276b^{b.sponge.V2.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0305007

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.mir-276b.sponge.V2}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fulga et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-mCherry.mir-276b.sponge.V2}

Cytology

Description

The P{UAS-mCherry.mir-276a.sponge.V2} and P{UAS-mCherry.mir-276b.sponge.V2} constructs target mir-276a-3P and mir-276b-3P respectively. However, because the only difference between mir-276a-3P and mir-276b-3P is at position 10, and position 10 is in the sponge bulge (not complementary to the miRNA), both sponges target both miRNAs.

(Cook and Van Vactor, 2015.6.15)

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

(Fulga et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Tagged with

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PG

(Li and Gavis, 2022)

viable, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

Phenotype Manifest In

dendrite | larval stage, with Scer\GAL4^ppk.PG

(Li and Gavis, 2022)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PG

(Li and Gavis, 2022)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, mir-276b^{b.sponge.V2.UAS.mCherry} has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^ppk.PG/nej^HMS01507

(Li and Gavis, 2022)

Suppressor of

Statement

Reference

mir-276b^{b.sponge.V2.UAS.mCherry}, Scer\GAL4^ppk.PG is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Fmr1^UAS.cZa, Scer\GAL4^ppk.PG

(Li and Gavis, 2022)

Scer\GAL4^ppk.PG/mir-276b^{b.sponge.V2.UAS.mCherry} is a suppressor of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^ppk.PG, nej^HMS01507

(Li and Gavis, 2022)

Other

Statement

Reference

Scer\GAL4^Cg.PA, mir-276a^{b.sponge.V2.UAS.mCherry}, mir-276b^{b.sponge.V2.UAS.mCherry} has decreased rate of adult locomotory behavior phenotype

(Tang et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, mir-276b^{b.sponge.V2.UAS.mCherry} has larval multidendritic class IV neuron phenotype, suppressible by Scer\GAL4^ppk.PG/nej^HMS01507

(Li and Gavis, 2022)

Scer\GAL4^ppk.PG, mir-276b^{b.sponge.V2.UAS.mCherry} has dendrite | larval stage phenotype, suppressible by Scer\GAL4^ppk.PG/nej^HMS01507

(Li and Gavis, 2022)

Suppressor of

Statement

Reference

mir-276b^{b.sponge.V2.UAS.mCherry}, Scer\GAL4^ppk.PG is a suppressor | partially of larval multidendritic class IV neuron phenotype of Fmr1^UAS.cZa, Scer\GAL4^ppk.PG

(Li and Gavis, 2022)

mir-276b^{b.sponge.V2.UAS.mCherry}, Scer\GAL4^ppk.PG is a suppressor | partially of dendrite | larval stage phenotype of Fmr1^UAS.cZa, Scer\GAL4^ppk.PG

(Li and Gavis, 2022)

Scer\GAL4^ppk.PG/mir-276b^{b.sponge.V2.UAS.mCherry} is a suppressor of larval multidendritic class IV neuron phenotype of Scer\GAL4^ppk.PG, nej^HMS01507

(Li and Gavis, 2022)

Scer\GAL4^ppk.PG/mir-276b^{b.sponge.V2.UAS.mCherry} is a suppressor of dendrite | larval stage phenotype of Scer\GAL4^ppk.PG, nej^HMS01507

(Li and Gavis, 2022)

Other

Statement

Reference

Scer\GAL4^Cg.PA, mir-276a^{b.sponge.V2.UAS.mCherry}, mir-276b^{b.sponge.V2.UAS.mCherry} has adult heart phenotype

(Tang et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

61407

w^*; P{UAS-mCherry.mir-276b.sponge.V2}attP40/CyO; P{UAS-mCherry.mir-276b.sponge.V2}attP2

Notes on Origin

Discoverer

Comments

Comments

The lack of a Scer\GAL4^tub.PU-mediated viability phenotype is a false negative (compared to independent viability data from miRNA deletion mutants).

(Fulga et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-276b^{b.sponge.V2.Scer\UAS.T:Disc\RFP-mCherry}

mir-276b^{b.sponge.V2.UAS.mCherry}

mir-276b^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections