UASt regulatory sequences drive expression of a full-length CG45075 open reading frame (ORF) which is tagged at the C-terminal end with three copies of Tag:HA. The D. melanogaster ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or the C-terminal tag sequence.
Expression of CG45075Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in partial female lethality at 25[o]C. The adult flies that are seen exhibit almost complete wing loss.