mir-965 mRNA has been inserted into the attP sites in mir-965KO2.
158 bp region containing the mir-965 hairpin was for rescue. The position of the rescue fragment on the reference sequence was determined from reported primer sequences.
mir-965KO2-Rescue flies do not display the abdominal segmentation phenotypes seen in mir-965KO2 mutants. As in wild type, histoblast division occurs synchronously and very little cell death is observed. The rate of histoblast nest expansion is similar to controls.