Deletion in Dlg5 due to imprecise excision of P{SUPor-P}Dlg5KG00748 resulting in a mutant that lacks the coding sequences for the N-terminal part of the protein.
When clones are induced in newly eclosed females and analyzed 10 days later, persistent stem cell-derived clones of both germline and somatic origin can be observed in wild-type females. In contrast, no persistent Dlg55D48 clones in the germline or follicle cells can be found under the same conditions. The percentage of ovarioles with transient wild-type and Dlg55D48 mutant clones are similar after 5 and days of clone induction. However, Dlg55D48 mutant clones are smaller than control clones.
Dlg55D48 embryos show defects in gonad formation. Dlg55D48 embryos show variable germ cell migration defects and germ cell loss. By stage 12 of embryogenesis, germ cell migration defects become apparent, as in a subset of Dlg55D48 mutant embryos, and primordial germ cells (PGCs) fail to align with somatic gonadal precursor cells (SGPs). Consequently, several PGCs are seen scattered in the posterior. Eventually, these PGCs are unable to establish and/or maintain sustained contacts with the SGPs and are detected several diameters away from the coalesced gonads. Fifty-six percent of Dlg55D48 embryos show five or more scattered PGCs compared with seventeen percent in controls.
