UAS regulatory sequences drive expression of Tag:HA-tagged caup, in which the cyc binding site has been deleted (amino acids 365-367) and the IRO-box has been mutated (amino acid replacements K459A and K461A).
Expression of caupcyc.IRO-box.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4salm.EPv results in a reduction in wing area that is no more severe than in caupIRO-box.Scer\UAS.T:Ivir\HA1.