FB2026_02 , released June 18, 2026
Allele: Hsap\LIN28B20xUAS.Tag:FLAG,Tag:HA
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General Information
Symbol
Hsap\LIN28B20xUAS.Tag:FLAG,Tag:HA
Species
H. sapiens
Name
FlyBase ID
FBal0317077
Feature type
allele
Associated gene
Hsap\LIN28B
Associated Insertion(s)
Carried in Construct
P{20XUAS-hsLIN28B.FLAG-HA}
Key Links
Transgenic product class
wild_type
(Chen et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Chen et al., 2015)
Mutagen
in vitro construct
(Chen et al., 2015)
Progenitor genotype
Carried in construct
P{20XUAS-hsLIN28B.FLAG-HA}
Cytology
Description

20xUAS regulatory sequences drive expression of Hsap\LIN28B which is tagged at the C-terminal end with three copies of Tag:FLAG and then three copies of Tag:HA.

(Chen et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\LIN28B
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing Hsap\LIN28B20xUAS.Tag:FLAG,Tag:HA under the control of Scer\GAL4αTub84B.PL results in a severely delayed onset of pupariation compared to wild-type controls.

      Expressing Hsap\LIN28B20xUAS.Tag:FLAG,Tag:HA under the control of Scer\GAL4Act5C.PI has no impact on pupal volume compared to wild-type controls.

      (González-Itier et al., 2018)

      Expression of Hsap\LIN28B20xScer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4esg.PU in adult intestinal stem cells (ISCs) and enteroblasts for two days (using the temperature sensitive Scer\GAL80ts.αTub84B line to control the timing of expression) does not result in a significant change in the total number of ISCs in the posterior midgut compared to in controls.

      (Chen et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\LIN28B20xScer\UAS.T:Zzzz\FLAG,T:Ivir\HA1
      Hsap\LIN28B20xUAS.Tag:FLAG,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)