A 6.15kb genomic fragment which encompasses the zpg transcription unit and an additional 1.5kb upstream and downstream of the locus. The zpg coding region is tagged at the C-terminal end with a short linker sequence (LAAA) followed by a GFP tag.
The expression of zpgT:Avic\GFP is able to fully rescue the spermatogenesis phenotype of zpgz-5352/zpgz-2533 and restore fertility to wild-type levels.