FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sgt56
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General Information
Symbol
Dmel\Sgt56
Species
D. melanogaster
Name
FlyBase ID
FBal0318419
Feature type
allele
Associated gene
Dmel\Sgt
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Uytterhoeven et al., 2015)
Progenitor genotype
P{SUPor-P}SgtKG04982
(Uytterhoeven et al., 2015)
Cytology
Description

Imprecise excision of the progenitor P{SUPor-P}SgtKG04982 insertion, resulting in a deletion that removes part of Sgt.

(Uytterhoeven et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Sgt56 larvae show a significant increase in endosomal microautophagy at the neuromuscular junction compared to wild-type controls. Ultrastructurally, the neuromuscular junctions of mutant larvae show a significant increase in the number of multivesicular bodies (MVBs) per bouton area and a significant increase in the number of intraluminal vesicles per MVB area.

      The mean excitatory junctional current (EJC) amplitude at the neuromuscular junction is significantly higher than wild type in Sgt56 larvae.

      (Uytterhoeven et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Sgt56 ; Hsc70-4Δ19/Df(3R)BSC471 double mutant larvae show the same decreased level of endosomal microautophagy and significant decrease in mean excitatory junctional current amplitude at the neuromuscular junction as seen in Hsc70-4Δ19/Df(3R)BSC471 single mutants.

      The increase in the number of multivesicular bodies (MVBs) and in the number of intraluminal vesicles per MVB area seen at the neuromuscular junction in Sgt56 larvae is suppressed in Sgt56 ; Hsc70-4Δ19/Df(3R)BSC471 double mutant larvae.

      (Uytterhoeven et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Sgt56 is rescued by Sgtwt.Tag:HA

      (Uytterhoeven et al., 2015)
      Comments

      Expression of Sgtwt.T:Ivir\HA1 completely rescues the increase in endosomal microautophagy which is seen at the neuromuscular junction in Sgt56 larvae.

      Expression of Sgtwt.T:Ivir\HA1 completely rescues the increase in mean excitatory junctional current amplitude which is seen at the neuromuscular junction in Sgt56 larvae.

      (Uytterhoeven et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sgt56
      sgt56
      (Uytterhoeven et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)