FlyBase Allele Report: Dmel\ppk26[Deg.UAS.mCherry]

General Information

Symbol

Dmel\ppk26^{Deg.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0318620

Feature type

allele

Associated gene

Dmel\ppk26

Associated Insertion(s)

Carried in Construct

P{UAS-ppk26.Deg.mCherry}

Key Links

Transgenic product class

missense_variant

(Gorczyca et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Gorczyca et al., 2014)

Mutagen

in vitro construct

(Gorczyca et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-ppk26.Deg.mCherry}

Cytology

Description

UAS regulatory sequences drive expression of a mutated form of ppk26 which carries the 'Degenerin' mutation (amino acid replacement A547V) in the peri-TM2 region of the protein. The protein is tagged at the C-terminal end with mCherry.

(Gorczyca et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ppk26

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | second instar larval stage, with Scer\GAL4^ppk.PG

(Gorczyca et al., 2014)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PG

(Gorczyca et al., 2014)

Phenotype Manifest In

larval multidendritic class IV neuron | second instar larval stage, with Scer\GAL4^ppk.PG

(Gorczyca et al., 2014)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PG

(Gorczyca et al., 2014)

Detailed Description

Statement

Reference

Overexpression of ppk26^{Deg.Scer\UAS.T:Disc\RFP-mCherry} driven by Scer\GAL4^ppk.PG leads to a reduction in dendritic arbors of class IV dendritic arborizing neurons in second or third instar larvae.

(Gorczyca et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ppk26^DEG

(Mitchell et al., 2023)

ppk26^{Deg.Scer\UAS.T:Disc\RFP-mCherry}

ppk26^{Deg.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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