UAS regulatory sequences drive expression of smo which carries phospho-mimetic mutations in the three PKA/CK1 phosphorylation sites (SD123), in the two Gprk2 sites (GPSD) and in the 'CL-II' Ser/Thr cluster in the juxtamembrane region of the C-terminal intracellular tail (carries the amino acid replacements S626D, S627D, S633D and S634D). The protein is tagged with CFP.