The conserved phenylalanine residue in each FP4 motif of the Zzzz\actA fragment has been replaced by alanine.
Embryos expressing Zzzz\actAAP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav.PLu do not show a significant ISNb bypass phenotype.
Embryos derived from females expressing Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 do not show lethality.
Expression of Zzzz\actAAP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4e22c does not result in embryonic lethality.