FlyBase Allele Report: Oari\PrP[VRQ.UAS]

General Information

Symbol

Oari\PrP^VRQ.UAS

Species

O. aries

Name

FlyBase ID

FBal0321257

Feature type

allele

Associated gene

Oari\PrP

Associated Insertion(s)

Carried in Construct

M{UAS-ovPrP.VRQ.RFP-}

M{UAS-ovPrP.VRQ}

Also Known As

VRQ(GPI), UAS-PrP, VRQ

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Thackray et al., 2012)

Progenitor genotype

Carried in construct

M{UAS-ovPrP.VRQ.RFP-}

M{UAS-ovPrP.VRQ}

Cytology

Description

UASt regulatory sequences drive expression of mature length Oari\PrP (amino acid residues 25-231) encoding the naturally occurring "VRQ" polymorphic variant (Val[136] Leu[141] Arg[154] Gln[171]). The protein is flanked at the N-terminal end by an insect secretion signal peptide and at the C-terminal end by the Oari\PrP glycosylphosphatidylinositol anchor signal sequence.

(Thackray et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Oari\PrP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of prion disease

CEA

(Avar et al., 2022, Thackray et al., 2020, Thackray et al., 2018, Thackray et al., 2012)

model of scrapie

CEA

(Thackray et al., 2014)

model of Creutzfeldt-Jakob disease

CEA

(Thackray et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Arctic.Aβ42.UAS.cJa.Tag:SS(nec)}

(Younan et al., 2018)

modeled by Hsap\APP^{Aβ40.UAS.cSa.Tag:SS(nec)}

(Younan et al., 2018)

modeled by Hsap\APP^{Aβ42.UAS.cSa.Tag:SS(nec)}

(Younan et al., 2018)

model of Creutzfeldt-Jakob disease

is exacerbated by DnaJ-1^HMS00688

(Thackray et al., 2022)

is ameliorated by DnaJ-1^UAS.cKa

(Thackray et al., 2022)

is exacerbated by Hsp110^HMS01080

(Thackray et al., 2022)

is ameliorated by Hsp110^UAS.cKa

(Thackray et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Zzzz\PrP^VRQ.Scer\UAS flies are exposed to (fed) ovine scrapie prion inocula during the larval stage in order to model prion disease. These flies show shortened survival time compared to controls, though no significant reduction in locomotor activity is observed.

(Thackray et al., 2012)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive | conditional, with Scer\GAL4^elav-C155

(Thackray et al., 2018)

short lived, with Scer\GAL4^elav-C155

(Thackray et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Unlike controls, flies expressing Oari\PrP^VRQ.UAS under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Act5C.PI and infected at the larval stage with PG127 or Apl[[338]] prions support prion propagation and support additional fly-to-mouse prion propagation; these flies also show a progressive decrease in climbing ability, which is much more severe than in infected controls. There is also fly-to-fly-to-mouse propagation of PG127 prions in the Scer\GAL4^elav-C155m driver background.

(Thackray et al., 2018)

The expression of Oari\PrP^VRQ.Scer\UAS under the control of Scer\GAL4^elav-C155 results in a decrease in adult lifespan compared to controls.

(Thackray et al., 2012)

Adults expressing Zzzz\PrP^VRQ.Scer\UAS under the control of Scer\GAL4^elav-C155 and exposed to VRQ/VRQ scrapie-infected sheep brain homogenate at the larval stage by oral exposure show a do not show an accelerated decline in locomotor activity with age compared to animals expressing Zzzz\PrP^VRQ.Scer\UAS under the control of Scer\GAL4^elav-C155 and exposed to control (scrapie-negative) VRQ/VRQ sheep brain material.

Adults expressing Zzzz\PrP^VRQ.Scer\UAS under the control of Scer\GAL4^elav-C155 and exposed to VRQ/VRQ scrapie-infected sheep brain homogenate at the larval stage by oral exposure show a significantly enhanced mortality rate compared to animals expressing Zzzz\PrP^VRQ.Scer\UAS under the control of Scer\GAL4^elav-C155 and exposed to control (scrapie-negative) VRQ/VRQ sheep brain material.

(Thackray et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Oari\PrP^VRQ.Scer\UAS

Oari\PrP^VRQ.UAS

Zzzz\PrP^VRQ.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

FBal0320643
FBal0282641

References (10)

Report Sections

Open Close