A heat-shock promoter regulates expression of Blimp-1 lacking the PEST-like sequences (aa 547-750) that are thought to modulate its protein degradation.
Using the heat-inducible Blimp-1ΔPEST.hs.T:Zzzz\FLAG, pupation timing is significantly delayed (increase in delay compared to induction of Blimp-1hs.T:Zzzz\FLAG), and around 40% fail to pupate after heat shock at 5hr APF.