FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\VPS35L774M.UAS.Tag:HA
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General Information
Symbol
Hsap\VPS35L774M.UAS.Tag:HA
Species
H. sapiens
Name
FlyBase ID
FBal0321388
Feature type
allele
Associated gene
Hsap\VPS35
Associated Insertion(s)
Carried in Construct
P{UAS-hVPS35.L774M.HA}
Key Links
Transgenic product class
missense_variant
(Wang et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2014)
Mutagen
in vitro construct
(Wang et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-hVPS35.L774M.HA}
Cytology
Description

UASt regulatory sequences drive expression of Hsap\VPS35 carrying the amino acid replacement L774M. The protein is tagged at the C-terminal end with Tag:HA.

(Wang et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\VPS35
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Wang et al., 2014)
      VPS35:p.Leu774Met
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: VPS35_487678
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\VPS35L774M.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Ddc.PU does not affect numbers of dopaminergic neurons in the adult brain (not even in 60-day-old flies) compared to controls. However, unlike in wild-type, the climbing ability of Hsap\VPS35L774M.Scer\UAS.T:Ivir\HA1-expressing adult flies does not decline much with age and is significantly better than in controls at 60 days post-eclosion. The median adult lifespan of Hsap\VPS35L774M.Scer\UAS.T:Ivir\HA1-expressing flies is significantly lower than in controls.

      (Wang et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\VPS35L774M.Scer\UAS.T:Ivir\HA1
      Hsap\VPS35L774M.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)