FlyBase Allele Report: Dmel\dom[B.Tag:TY1,sfGFP,Tag:FLAG]

General Information

Symbol

Dmel\dom^{B.Tag:TY1,sfGFP,Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0322591

Feature type

allele

Associated gene

Dmel\dom

Associated Insertion(s)

Carried in Construct

P{dom-B-GFP}

Also Known As

dom-B-GFP

Key Links

Transgenic product class

genomic_DNA

(Börner and Becker, 2016)

wild_type

(Börner and Becker, 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Börner and Becker, 2016)

wild_type

(Börner and Becker, 2016)

Mutagen

in vitro construct

(Börner and Becker, 2016)

Progenitor genotype

Carried in construct

P{dom-B-GFP}

Cytology

Description

Genomic fragment (derived from fosmid library clone pflyfos016675) encompassing the dom gene. The dom B isoform is tagged at the C-terminal end with a 2xTY1-sGFP-3xFLAG tag (isoform B corresponds to the protein encoded by the DOM-RE transcript, FlyBase release FB2016_03).

(Börner and Becker, 2016)

Allele components

Component

Use(s)

Regulatory region(s)

dom

Encoded product / tool

dom

Tagged with

sfGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

female germline cell

(Börner and Becker, 2016)

female germline stem cell

(Börner and Becker, 2016)

somatic cell of ovariole

(Börner and Becker, 2016)

follicle cell

(Börner and Becker, 2016)

oocyte

(Börner and Becker, 2016)

nurse cell

(Börner and Becker, 2016)

adult stage | female & oogenesis

cystoblast

(Börner and Becker, 2016)

follicle stem cell

(Börner and Becker, 2016)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

dom^{B.Tag:TY1,sfGFP,Tag:FLAG}/Scer\GAL4^{VP16.mat.αTub67C} partially rescues dom^HMS02208

(Ibarra-Morales et al., 2021)

dom^{B.Tag:TY1,sfGFP,Tag:FLAG} partially rescues dom^k08108

(Börner and Becker, 2016)

dom^{B.Tag:TY1,sfGFP,Tag:FLAG} partially rescues dom⁹

(Börner and Becker, 2016)

Comments

Expression of dom^{B.T:Scer\TY1,T:Avic\GFP-SF,T:Zzzz\FLAG} partially rescues the pupal lethality of dom⁹/dom⁹ mutants, and surviving females do not exhibit a significant decrease in fertility.

Expression of dom^{B.T:Scer\TY1,T:Avic\GFP-SF,T:Zzzz\FLAG} partially rescues the pupal lethality and larval lymph gland necrosis seen in dom^k08108/dom^k08108 mutants.

(Börner and Becker, 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dom^{B.T:Scer\TY1,T:Avic\GFP-SF,T:Zzzz\FLAG}

dom^{B.Tag:TY1,sfGFP,Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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