FB2026_03 , released September 17, 2026
Allele: Spyo\Cas9dCas9-VP64.UAS.Tag:FLAG
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General Information
Symbol
Spyo\Cas9dCas9-VP64.UAS.Tag:FLAG
Species
S. pyogenes
Name
FlyBase ID
FBal0322767
Feature type
allele
Associated gene
Spyo\Cas9
Associated Insertion(s)
Carried in Construct
P{UAS-3xFLAG.dCas9.VP64}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Lin et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-3xFLAG.dCas9.VP64}
Cytology
Description

10x UAS regulates expression of nuclease-dead Spyo\Cas9 (dCas9, containing two nuclease-attenuating mutations: D10A and H840A) tagged at the N-terminus with 3x Tag:FLAG and a nuclear localization sequence (NLS), and fused to a second NLS followed by a VP64 activator domain at the C-terminus. The protein produced is unable to activate expression of an endogenous gene of interest when combined with sgRNAs that target sequences upstream of the transcription start site of the gene of interest.

(Lin et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Spyo\Cas9
Tagged with
Tag:FLAG
Tag:NLS(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Spyo\Cas9d.Scer\UAS.T:Ivir\HA1,T:Uuuu\nls-Unk,T:Zzzz\VP64
      Spyo\Cas9d.Scer\UAS.T:Zzzz\FLAG,T:Uuuu\nls-Unk,T:Zzzz\VP64
      Spyo\Cas9dCas9-VP64.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)