JhI-1^ED24 mutant somatic clones induced in JhI-1^{ΔMTS.T:SV5\V5} expressing background are significantly smaller than their twin spot clone in third instar larval eye discs and clones located posterior of the morphogenetic furrow where a band of cells synchronously enter the final round of mitosis show fewer cells in the S-phase and no cells in the M-phase of the mitotic cycle.

JhI-1^ED24;JhI-1^{ΔMTS.T:SV5\V5} mutant cells derived from JhI-1^ED24 homozygous mutant somatic clones induced in wing discs carrying the JhI-1^{ΔMTS.T:SV5\V5} transgene as well as a Minute mutation (to give the JhI-1^ED24 mutant clones a growth advantage) show a significant delay in the G[[2]]/M transition of the mitotic cell cycle compared to controls and a sharp increase in the production of reactive oxygen species (ROS) and DNA damage markers. However, no induction of apoptosis is observed in these clones either in the wing or the antenna imaginal discs. The JhI-1^ED24;JhI-1^{ΔMTS.T:SV5\V5} wing disc cells have normal size.