A 13.2kb genomic fragment containing the per gene, into which the phosphomimetic S826D and S828D mutations have been introduced. The open reading frame is tagged at the C-terminal end with Tag:HA and multiple histidine residues (10xHis).
The per01;perS826D.S828D.T:Ivir\HA1,T:Zzzz\His6 flies show defects in the temperature compensation of period length: their free-running locomotor activity periods lengthen with the rising temperature.