FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Mmus\McuD260Q.E263Q.UAS.Tag:FLAG
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General Information
Symbol
Mmus\McuD260Q.E263Q.UAS.Tag:FLAG
Species
M. musculus
Name
FlyBase ID
FBal0324817
Feature type
allele
Associated gene
Mmus\Mcu
Associated Insertion(s)
Carried in Construct
P{UAS-mMcu.D260Q.E263Q}
Transgenic product class
dominant_negative_variant
(Drago and Davis, 2016)
missense_variant
(Drago and Davis, 2016)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Drago and Davis, 2016)
missense_variant
(Drago and Davis, 2016)
Mutagen
in vitro construct
(Drago and Davis, 2016)
Progenitor genotype
Carried in construct
P{UAS-mMcu.D260Q.E263Q}
Cytology
Description

UAS regulatory sequences drive expression of a dominant negative form of Mmus\Mcu (carries the mutations D260Q and E263Q). The protein is tagged with Tag:FLAG.

(Drago and Davis, 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mmus\Mcu
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Mmus\McuD260Q.E263Q.Scer\UAS.T:Zzzz\FLAG, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR13F02 either unconstrained or Gal80[ts]-limited expression to pre-adult developing stages, but not to adulthood, impairs adult olfactory memory, as compared to controls; unconstrained expression impairs intermediate-term memory, rather than olfactory or shock avoidance or learning.

      Expression of Mmus\McuD260Q.E263Q.Scer\UAS.T:Zzzz\FLAG, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR13F02 impairs adult olfactory intermediate-term memory in olfactory aversive tests (but not olfactory avoidance, shock avoidance or learning) as compared to controls; expression of Mmus\McuD260Q.E263Q.Scer\UAS.T:Zzzz\FLAG, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR13F02 and temporally-limited by Gal80[ts] to pre-adult developing stages, but not to adulthood, impairs adult olfactory intermediate-term memory in olfactory aversive tests (but not olfactory avoidance, shock avoidance or learning) as compared to controls.

      Expression of Mmus\McuD260Q.E263Q.Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4GMR25H11 does not impair odor avoidance or shock avoidance.

      (Drago and Davis, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mmus\McuD260Q.E263Q.Scer\UAS.T:Zzzz\FLAG
      Mmus\McuD260Q.E263Q.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)