FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\TARDBPAggIn.UAS.Tag:FLAG
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General Information
Symbol
Hsap\TARDBPAggIn.UAS.Tag:FLAG
Species
H. sapiens
Name
FlyBase ID
FBal0325754
Feature type
allele
Associated gene
Hsap\TARDBP
Associated Insertion(s)
Carried in Construct
M{UAS-hTARDBP.AggIn.FLAG}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Langellotti et al., 2016)
Progenitor genotype
Carried in construct
M{UAS-hTARDBP.AggIn.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of a chimeric Hsap\TARDBP protein that consists of the N-terminal 100 amino acids (this contains the nuclear localization sequence), the linker region that is normally found between the two RNA recognition motifs (RRMs), a mutated form of RRM2 that cannot bind RNA but retains the nuclear export sequence, and 12 tandem copies of its prion-like Q/N-rich region. The protein is tagged at the N-terminal end with Tag:FLAG.

(Langellotti et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\TARDBP
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  amyotrophic lateral sclerosis
      CEA
      (Langellotti et al., 2016)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Hsap\TARDBPAggIn.Scer\UAS.T:Zzzz\FLAG have significantly shorter adult lifespan and along with age-progressive climbing defects (with the Scer\GAL4elav.PU driver) but do not display any obvious eye morphological defects (with the Scer\GAL4GMR.PU driver), not even during aging, although they show slightly impaired phototaxis relative to controls.

      Depending on the particular insertion Hsap\TARDBPAggIn.Scer\UAS.T:Zzzz\FLAG-expressing (under Scer\GAL4elav.PU) flies display less or more severe increase in larval or pupal lethality and with a strong insertion line (5A), the larvae (3rd instar) also show reduced motility (number of peristaltic waves per unit of time) compared to controls.

      (Langellotti et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\TARDBPAggIn.Scer\UAS.T:Zzzz\FLAG
      Hsap\TARDBPAggIn.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)