UASt regulatory sequences drive expression of wild-type Spt5 that is tagged at the C-terminal end with two tandem copies of Tag:FLAG. Synonymous mutations have been introduced into the sequence so that it is resistant to the Spt5HMS00153 RNAi line.
Scer\GAL4wor.PA/Spt5UAS.Tag:FLAG is an enhancer of short lived phenotype of Scer\GAL4wor.PA, bratHMS01121
The co-expression of Spt5Scer\UAS.T:Zzzz\FLAG rescues the lethality induced by the expression of Spt5HMS00153 under the control of Scer\GAL4Act5C.PI.