A sub-region of the nerfin-1 3'UTR (852bp, contains the mir-92a, mir-92b, mir-310-mir-313, mir-279/mir-286, mir-34, mir-315, mir-305, mir-307a, mir-5, mir-13b-1 and mir-13b-2 miRNA-binding sites) has been inserted into a Not-I site within the SV40 3'UTR of Avic\GFPEGFP.nerfin-1.SV40.Tag:NLS(tra), 14bp 3' of the EGFP ORF translation stop codon.