A sub-region of the nerfin-1 3'UTR (32bp, contains the overlapping mir-279 and mir-286 miRNA-binding sites) has been inserted into a Not-I site within the SV40 3'UTR of Avic\GFPEGFP.nerfin-1.SV40.Tag:NLS(tra), 14bp 3' of the EGFP ORF translation stop codon.