A sub-region of the nerfin-1 3'UTR (138bp, contains the overlapping mir-34/mir-315/mir-305 and mir-307a miRNA-binding sites) has been inserted into a Not-I site within the SV40 3'UTR of Avic\GFPEGFP.nerfin-1.SV40.Tag:NLS(tra), 14bp 3' of the EGFP ORF translation stop codon.