A sub-region of the nerfin-1 3'UTR (29bp, contains a predicted mir-5 miRNA-binding site) has been inserted into a Not-I site within the SV40 3'UTR of Avic\GFPEGFP.nerfin-1.SV40.Tag:NLS(tra), 14bp 3' of the EGFP ORF translation stop codon.