FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\gnu9A.GFP
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General Information
Symbol
Dmel\gnu9A.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0327523
Feature type
allele
Associated gene
Dmel\gnu
Associated Insertion(s)
Carried in Construct
P{gnu-GFP.9A}
Key Links
Transgenic product class
missense_variant
(Hara et al., 2017)
polypeptide_post_translational_processing_variant
(Hara et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Hara et al., 2017)
polypeptide_post_translational_processing_variant
(Hara et al., 2017)
Mutagen
in vitro construct
(Hara et al., 2017)
Progenitor genotype
Carried in construct
P{gnu-GFP.9A}
Cytology
Description

Genomic sequence of gnu (around 3kb, including regulatory elements), with GFP (plus a linker GGCGGAAGTGGAGCGGCCGCC sequence) inserted into the 3' of the gnu ORF; the gnu sequence is a phosphomutant version that is not phosphorylated by CycB/Cdk1.

(Hara et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
gnu
Encoded product / tool
gnu
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      gnu305 mutant females lay embryos that have few giant nuclei (due to inability to undergo mitosis) while embryos from gnu305 mothers carrying the gnu9A.T:Avic\GFP construct show only partial rescue of the phenotype: the embryos undergo mitosis but most show defects in early cycles or in blastoderm stage.

      The progression through meiosis upon egg activation is delayed (condensed phase of metaphase I persists longer than in wild-type and the metaphase I/anaphase I transition is delayed) in gnu305;gnu9A.T:Avic\GFP oocytes, the morphology of chromosomes at metaphase I is frequently aberrant and defects in meiotic chromosome segregation are also observed.

      (Hara et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      gnu9A.GFP
      gnu9A.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)