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General Information
Symbol
Scer\FCY1::Scer\FUR1n.UAS.Tag:HA
Species
S. cerevisiae
Name
FlyBase ID
FBal0336330
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a fusion protein containing both the Scer\FCY1 (a cytosine deaminase) and Scer\FUR1 (a uracil phosphoribosyltransferase) coding regions. The coding sequence has not been codon-optimized for Drosophila. The fusion protein is tagged at the N-terminal end with Tag:HA. This fusion protein is designed to be used in the 'EC-tagging' technique: when flies are exposed to 5-ethynylcytosine (EC), the sequential activity of the two enzymes encoded by the fusion protein converts the 5-ethynylcytosine into 5-ethynyluridine monophosphate that is subsequently incorporated into nascent RNAs. The ethynyl group allows detection and purification of the tagged RNAs.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\FCY1::Scer\FUR1n.Scer\UAS.T:Ivir\HA1
Scer\FCY1::Scer\FUR1n.UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (1)