Insertion of a genomic Cirl fragment at the attP site in CirlKO.attP.w-; the fragment contains an mRFP(Unk) cassette inserted into an exon encoding part of the extracellular domain (ECD) and a T>A mutation in the GPCR autoproteolysis inducing (GAIN) domain (to abolish autoproteolytic activity); also contains a w+* marker.