FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\FicE247G.UAS.Tag:V5.Tag:polyHis
Open Close
General Information
Symbol
Dmel\FicE247G.UAS.Tag:V5.Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0337974
Feature type
allele
Associated gene
Dmel\Fic
Associated Insertion(s)
Carried in Construct
PBac{UAS-Fic.E247G}
Also Known As
UAS-FicE247G
Key Links
Transgenic product class
missense_variant
(Casey et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Casey et al., 2017)
Mutagen
in vitro construct
(Casey et al., 2017)
Progenitor genotype
Carried in construct
PBac{UAS-Fic.E247G}
Cytology
Description

UAS regulatory sequences drive expression of a constitutively active form of Fic (carries the amino acid replacement E247G). The protein is tagged at the C-terminal end with Tag:V5 and Tag:polyHis.

(Casey et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fic
Tagged with
Tag:polyHis
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of FicE247G.Scer\UAS.T:SV5\V5.T:Zzzz\His6 under the control of either Scer\GAL4da.G32 or Scer\GAL4GMR.long in an otherwise wild-type background is viable and adults have no externally visible phenotypes. In an Fic30C homozygosity background, however, Scer\GAL4da.G32-driven expression results in lethality. In Fic30C homozygosity or Fic30C/Df(2L)BSC296 backgrounds, Scer\GAL4GMR.long-driven expression is viable but leads to a small and rough eye phenotype.

      The expression of FicE247G.Scer\UAS.T:SV5\V5.T:Zzzz\His6 under the control of Scer\GAL4GMR.long in an Fic30C homozygosity background, leads to a strong increase in apoptosis on the third instar larval eye disc, as compared to the expression of FicE247G.Scer\UAS.T:SV5\V5.T:Zzzz\His6 under the control of Scer\GAL4GMR.long or wild-type controls.

      (Casey et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      Fic30C homozygotes expressing FicE247G.Scer\UAS.T:SV5\V5.T:Zzzz\His6 under the control of Scer\GAL4GMR.long exhibit a small and rough eye phenotype, which is rescued by the co-expression of FicScer\UAS.T:SV5\V5.T:Zzzz\His6 or FicI271D.Scer\UAS.T:SV5\V5.T:Zzzz\His6, but not FicH375A.Scer\UAS.T:SV5\V5.T:Zzzz\His6.

      (Casey et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      FicE247G.Scer\UAS.T:SV5\V5.T:Zzzz\His6
      FicE247G.UAS.Tag:V5.Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (3)