FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\FicE247G.I271D.UAS.Tag:V5.Tag:polyHis
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General Information
Symbol
Dmel\FicE247G.I271D.UAS.Tag:V5.Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0337977
Feature type
allele
Associated gene
Dmel\Fic
Associated Insertion(s)
Carried in Construct
PBac{UAS-Fic.E247G.I271D}
Key Links
Transgenic product class
missense_variant
(Casey et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Casey et al., 2017)
Mutagen
in vitro construct
(Casey et al., 2017)
Progenitor genotype
Carried in construct
PBac{UAS-Fic.E247G.I271D}
Cytology
Description

UAS regulatory sequences drive expression of Fic carrying the mutations E247G (this mutation results in a constitutively active form of Fic when present by itself) and I271D (this mutation disrupts dimerization). The protein is tagged at the C-terminal end with Tag:V5 and Tag:polyHis.

(Casey et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fic
Tagged with
Tag:polyHis
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of FicE247G.I271D.Scer\UAS.T:SV5\V5.T:Zzzz\His6 under the control of Scer\GAL4GMR.long alone is viable and adults have no externally visible phenotypes; in an Fic30C homozygosity background, however, this co-expression leads to lethality.

      (Casey et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      FicE247G.I271D.Scer\UAS.T:SV5\V5.T:Zzzz\His6
      FicE247G.I271D.UAS.Tag:V5.Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (1)